Biosynthesis of Polysaccharides in Acetobacter xylinum
2005
The sequential synthesis in vitro of a heptasaccharide diphosphate prenol, containing glucose, mannose, glucuronic acid and rhamnose in the ratio 4:1:1:1 is described. The enzyme preparation consisted of EDTA-treated Acetobacter xylinum cells and UDP-glucose, GDO-mannose, UDP-glucuronic acid and TDP-rhamnose were employed as sugar donors.
The compounds soluble in chloroform/methanol/water (1:2:0.3) formed from incubations carried out under different conditions in the presence of a variety of combinations of the donors labeled with 14C, 3H or 32P were analysed by DEAE-cellulose column chromatography, gel filtraton, partial acid hydrolysis, acetolysis, periodate oxidation, etc.
The following structure is proposed for the most complex compound characterized: rhamnosyL(1 6)-β-glucosyl-(1 6)-α-glucosyl-(1 4)-β-glucuronyl-(1 6)-β-mannosyl-(1 3)-β-glucosyl-(1 4)-α-glucosyl diphosphate prenol.
The smaller oligosaccharide diphosphate prenols formed as intermediate steps are also characterized in this or in previous work [Garcia, R. C., Recondo, E. and Dankert, M. A. (1974) Eur. J. Biochem. 43, 93–105; Couso, R. O., Ielpi, L., and Damkert, M. A. (1980) Arch. Biochem. Biophys. 204, 434–443].
The role of these compounds in the biosynthesis of a complex exopolysaccharide that this microorganism forms in addition to cellulose is discussed.
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