Effects of dental adhesives on micronucleus frequency in peripheral blood lymphocytes in vitro.

Dental adhesives come into direct contact with oral tissues. Due to this close and long-term contact, the materials should exhibit a high degree of biocompatibility. The aim of this study was to evaluate their genotoxic effect of dental adhesives on human lymphocytes in vitro. Polymerized dental adhesives (Excite, Adper Single Bond 2, Prompt L-pop and OptiBond Solo Plus) were eluted in a dimethyl sulfoxide for 1 hour, 1 day, and 5 days. Thereafter lymphocyte cultures were treated with different concentrations of eluates (0.2 µg/ml, 0.5 µg/ml and 5 µg/ml) obtained from each of the tested materials. Genotoxiciy was evaluated by micronucleus test. Chi square test was used for the statistical analysis (p<0.05). After treatment with 1 hour elution period only the highest dose of all tested materials affected the measured cytogenetic parameters, while after treatment with 1 day of elution period, genotoxicity was demonstrated in cultures treated with eluates in concentrations of 0.5 µg/ml and 5 µg/ml. Based on the results, we can conclude that the use of dental adhesives causes genotoxic effects in human lymphocytes. Toxic effect increases with a concentration of tested material and decreases with duration of elution period.