Quantitative analysis of five components in the rhizome of Angelica polymorpha by RP-HPLC under different UV wavelengths

A HPLC method was developed for simultaneouly quantitative analyses of aviprin (1), gosferol (2), 3′R-(+)-hamaudol (3), 3′-O-acetylhamaudol (4) and iso-imperatorin (5) in the rhizome of Angelica polymorpha. The analysis was performed at 25 ℃ on an Agilent Eclipse XDB-C18 analytical column (4.6 mm×250 mm, 5 μm) with the mobile phase of methanol and H2O in gradient elution [ 55∶45 (0 min)-65∶35 (25 min)-95∶5 (35 min) ]. And the flow rate was 1.0 mL min-1 with the detection wavelengths of 312 nm (1), 306 nm (2), 300 nm (3), 294 nm (4) and 310 nm (5). Consequently, the reqresion equations were Y=1.81×103X+7.93×102(r=0.999 6), Y=2.49×103 X-2.17×102 (r=0.999 3), Y=2.02 ×103X-1.42×102 (r=0.999 1), Y=1.57×103X-0.66×102 (r=0.999 7), Y=2.65×103X-1.47×102 (r=0.999 6). And the average recoveries were 99.7% (RSD 0.57%), 100.1% (RSD 1.3%), 100.0% (RSD 1.6%), 99.6% (RSD 1.3%), 99.2% (RSD 0.59%), respectively. The precision, repeatability and stability were all consistent with the request of quantitative analysis. The contents of compound 1-5 in A. polymorpha were determined as 0.525%, 0.044%, 0.046%, 0.043%, 0.15%, respectively. Accordingly, this quantitative analysis method is good for the quality control of A. polymorpha.