The Role of Autologous PRP on Deep Partial-Thickness Burn Wound Healing in Bama Pigs

In this study, we aimed to evaluate the therapeutic effects of autologous PRP on deep partial-thickness burns in Bama pigs. Deep partial-thickness burn wounds were created on the back of Bama pigs. The re-epithelialization time was compared between the PRP and control groups. The mean score of Ki67 (+) cells and alpha-SMA (+) vessels, the mean thickness of epidermis and dermis of the healing wounds were determined via HE staining and immunohistochemical assay. The levels of the growth factors EGF, bFGF and VEGF were examined by ELISA. Our data showed that the time to wound re-epithelialization was shorter in the PRP group compared to the control group. The thickness of the epidermis was larger in the PRP group compared to the control group. On the 7th and 14th days after the treatment, the mean score of Ki67 (+) cells and alpha-SMA (+) vessels were higher in the PRP group compared to the control group. The PRP group showed higher levels of growth factors (EGF, bFGF and VEGF) compared to the control group by ELISA. The results indicated that PRP could improve wound healing process of deep partial-thickness burns in Bama pigs. The PRP increased the thickness of epidermis of the healed wounds, cell proliferation and angiogenesis. We demonstrated that applying PRP had a greater potential for the treatment of deep partial-thickness burns.