THE BIOACTIVE ASSAY AND ELECTROPHORETIC ANALYSIS OF TWO MOLECULAR WEIGHT FRACTIONS FROM THE EXTRACTS OF SPINAL DORSAL HORN IN MORPHINE SPARED ROOT RAT

Objective The neurite\|promoting effects of two molecular weight fractions(10kD and 10kD) from the extracts of operated side and unoperated side spinal dorsal horn in spared root rat on dorsal root ganglion (DRG) of chick embryo were explored. The influence of the extracts from morphine spared root rat on the neurite\|promoting effects was also observed. Methods The neurotrophic active substances were isolated and analyzed by some methods of ultrafilter preparing the extract, tissue culture and SDS\|polyacrylamide gel electrophoresis (SDS\|PAGE). Results DRG neuritic density treated by molecular weight fraction of 10kD from the extract of operated side spinal dorsal horn in spared root rat was larger than that of10kD and 10kD from the extract of unoperated side, and of 10kD from the extract of operated side. The DRG neuritic density treated by molecular weight fraction of 10kD from the extract of operated side spinal dorsal horn in morphine spared root rat was again larger than that of operated side spinal dorsal horn in spared root rat. The number of electrophoretic zone of samples was approximately the same in operated side and unoperated side, whereas the percentage of absorptive peak area of three zones with approximate 67kD, 31kD and 16kD from operated side of two groups of animal was larger respectively in comparison with unoperated side. Conclusion Morphine might enhance the neurite\|promoting effect of the extract of 10kD fraction from deafferented spinal dorsal horn on DRG. The content of 67kD, 31kD and 16kD protein at 10kD fraction from operated side of two groups of animal was tending towards increasing, especially the approximate 67kD protein content from operated side of morphine group of animal.\;