Protease Inhibitor Associated Antimicrobial Activit y of Pea Pisum sativum L. cv. Arkel

Aqueous and organic extracts were prepared from fre sh pea seeds by standard methods. The extracts were analyzed for protease inhibitory acti vity using Dot,blot assay. The aqueous as well as the organic extracts obtained with ethanol showed s ignificant protease inhibitory activity. The aqueous extracts were treated with ammonium sulfate to cause differential protein precipitation. 30%, 60% and 90% ammonium sulfate saturations were used to prepare these different protein fractions. 60% and 90% fractions were positive for protease inhibitor (PI) activity on dot blot assay, the 30% fraction did not show any inhibitory activi ty. The assay of antibacterial activity by disc diffusion method using E.coli (ATCC 25922) strain r evealed that the crude aqueous extract, 90% fraction and ethanolic extract were highly active i n inhibiting the bacterial growth, the 60% fraction showed moderate antibacterial activity and the 30% fraction was not significant.