PRODUCTION OF PURIFIED FIBRINOGEN CONCENTRATE

Aim . To present a technique for obtaining fibrinogen concentrate purified from ballast proteins, which can be used in hemostatic drug production. Materials and methods. The proposed method is based on isolating fibrinogen from a cryoprecipitate solution by its precipitation with a polyethylene glycol solution followed by virus inactivation by a solvent/detergent. Purification from viral inactivation products and ballast proteins is performed by a two-step processing of the obtained fibrinogen concentrate with glycine solution. Results. The developed laboratory method for isolating fibrinogen has been optimized in terms of main parameters and scaled in pilot production. Conclusion. The presented laboratory technique for fibrinogen extraction is characterized by a high yield of the target product, thus being suitable for the production of hemostatic drugs.