ReSequencer: an automated primer picking application for gene resequencing efforts.

Summary: ReSequencer is a stream-lined, cross-platform, standalone application that is capable of retrieving the DNA sequence of a gene’s exons and then designing primers to sequence each exon. This application remotely accesses Ensembl to search retrieve the DNA sequence for any known transcript for a gene. Once the sequence for each exon is retrieved, ReSequencer runs a client-side binary of Primer3 to design primers based on the user’s parameters. During the course of resequencing over 100 genes related to lung cancer, we have found ReSequencer to significantly speed up this project by successfully automating primer selection for XX % of the XXX exons involved in the project. Availability: http://www.tgen.org/software/resequencer