Peptide-based protein capture agents with high affinity, selectivity, and stability as antibody replacements in biodetection assays
2014
Current biodetection assays that employ monoclonal antibodies as primary capture agents exhibit limited
fieldability, shelf life, and performance due to batch-to-batch production variability and restricted thermal stability. In
order to improve upon the detection of biological threats in fieldable assays and systems for the Army, we are
investigating protein catalyzed capture (PCC) agents as drop-in replacements for the existing antibody technology
through iterative in situ click chemistry. The PCC agent oligopeptides are developed against known protein epitopes and
can be mass produced using robotic methods. In this work, a PCC agent under development will be discussed. The
performance, including affinity, selectivity, and stability of the capture agent technology, is analyzed by
immunoprecipitation, western blotting, and ELISA experiments. The oligopeptide demonstrates superb selectivity
coupled with high affinity through multi-ligand design, and improved thermal, chemical, and biochemical stability due
to non-natural amino acid PCC agent design.
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