Identification and Characterization of a Novel Trans-membrane Protein Gene, pdh1, from Schizosaccharomyces pombe

We have cloned a new gene, pdhl, from genomic DNA of fission yeast Schizosaccharomyces pombe. pdhl is actively transcribed as 1400-nucleotide mRNA in vegetatively growing cells and can code for a 226 amino acid polypeptide (pdhlp). Computational structural prediction has revealed that the pdhlp is a highly hydrophobic protein with seven transmembrane domains. The prediction has also detected a possible C-kinase phosphorylation site within the longest hydrophilic loop.