Isolation of Tatumella ptyseos from Beef in Ibadan, Nigeria

SUMMARY Two hundred beef samples randomly collected from Bodija market in Ibadan, Nigeria were investigated bacteriologically. Four isolates resembling members of the Enterobacteriaceae family by phenotypic characterisation were further examined on MacConkey agar (MCA), Columbia agar with 5% ovine blood (CA) and biochemically. All the four isolates were identified as Tatumella ptyseos using the classical method and an online Advanced Bacterial Identification Software (ABIS). The susceptibility of the four isolates to antimicrobial agents was determined using the disk diffusion method. All the four isolates grew under aerobic conditions with no haemolysis on the CA supplemented with 5% ovine blood and as non-lactose fermentersion on MCA. Colonies were 0.5 - 1mm in diameter on CA with smooth and entire edge after 24hr incubation. The o isolates were Gram negative bacilli, motile at 25 C but not at o 37 C, oxidase negative, catalase positive, Simmon citrate