EPIGALLOCATECHIN GALLATE OF GREEN TEA INHIBITS PROLIFERATION, DIFFERENTIATION AND TNF-α IN THE PRIMARY HUMAN VISCERAL PREADIPOCYTES CULTURE

Objectives Green tea EGCG (epigallocathechin gallate) have shown to cause loss of body fat and also inhibit the growth of cancer cell. Therefore the aim of this study is to determine whether green tea EGCG can directly inhibit proliferation, differentiation and TNF-α (tumor necrosis factor-α) of the human visceral preadipocyte culture. Methods Primary human visceral pre adipocytes culture were treated with EGCG in several doses 0 μM (control group), 10 μM, 50 μM, 100 μM and 200 μM for 24 hours respectively. The human visceral tissues specimens were derived from the Surgery dept. of Dr. Syaiful Anwar General Hospital in Malang. Cellular account of the adipogenesis processes (proliferation, differentiated and undifferentiated cells) was determined using microscope after hematoxylline staining. TNF-α concentration derived from primary human preadipocytes culture also observed using ELISA Kit Assay design. Results It was found that there were a dose dependent respond to the cellular account of the adipogenesis processes. There were a significant (p=0.000) decrease of the cellular account for proliferation in EGCG control group (78.8 + 5.88%), EGCG 10 μM (66.32 + 4.63%), EGCG 50 μM (48.72+ 3.84%,), EGCG 100 μM (28.2+4.63%) and EGCG 200 μM (62.68+3.67%). There were also significant decrease of the percentages in the differentiated cells in EGCG control group (66.43+ 3.62%), EGCG 10μM (67.56+3.61%), EGCG 50 μM (62.75+2.42%), EGCG 100 μM (21.52+3.44%), EGCG 200 μM (28.04+1.24%). There were not any changes in the percentages of undifferentiation cell in the groups of EGCG 10 μM and 50 μM, however there was significant (p