Dichioromethylenediphosphonic tetraacid targets ischemic/reperfused myocardium

AbstractThe objective of this investigation was to examine the ability of two diphosphonate compounds, ethylenediaminetetraacetic acid (EDTA), and D-mannitol to specifically target ischemic/infarcted myocardium. Dichloromethylenephosphonic tetraester or tetraacid, EDTA, or D-mannitol (n=6 each group) was administered iv in intact animal preparations of experimentally induced acute myocardial ischemia. All four of the above compounds were radioactively labeled with 14C. Anesthetized, open-chest rabbits were instrumented for measurement of systemic hemodynamics and left ventricular pressure. Animals were subjected to 30 min of left main coronary artery occlusion (marginal branch) followed by 2 h of reperfusion. All test agents (25 μCi each) were administered 5 min prior to reperfusion via a previously implanted left atrial catheter. Following reperfusion, samples of infarcted myocardium and adjacent ischemic but still viable myocardium were identified for comparison to the normal zone. Among the four drug t...