1‐Phenyl‐3‐toluyl‐4‐ [ortho‐1′‐(N‐ethyl‐2′‐methylpropylamine)]phenylpyrazole, Synthesis and Evaluation of the in vitro Antifungal Activity Against Botrytis cinerea and Fusarium oxysporum.

Abstract A novel antifungal pyrazole derivative was synthesized. Designated 1-phenyl-3-toluyl-4-[ ortho -1′-( N -ethyl-2′-methylpropylamine)]phenylpyrazole, the compound exerted an antifungal effect toward Botrytis cinerea and Fusarium oxysporum . In fact, our results clearly show that mycelial growth and conidial germination of both fungi were blocked by the compound. Indeed, a 96-well microbioassay procedure was used for fast and easy evaluation of minimal inhibitory concentration (MIC). The MIC values for B. cinerea and F. oxysporum were 25 and 36 μg/ml, respectively.