Induction of extracellular protease and subtilisin-like protease of Beauveria bassiana.

Inocula and inducers with their effects on production of the total extracellular protease and subtilisin-like protease ( Pr 1 ) of Beauveria bassiana were studied. It was found that production of the total extracellular protease and Pr 1 was distinctly different among the inocula and varied between the inducers. Having been induced by cicada exuviae inducer [( ρ /g L -1 ): cicada exuviae 2, chitin 2, K 2HPO 4 0.3,NaCl 0.3, MgSO 4 .7H 2O 0.3,add 1 L water] for 12 h, germ tube produced greater amount of total extracellular protease as well as Pr 1 than other combination of inocula and inducers. The production of enzymes made by mycelia cultured on different media was different. In the inducing products, change of total extracellular protease and that of Pr 1 had the same tendency in content, but the former did not relate closely to the latter in activity. Fig 2, Tab 2, Ref 16