Time-Resolved Tracking of a Minimum Gene Expression System Reconstituted in Giant Liposomes

Individual expression: We describe a method that allows the observation of real-time gene expression in a large number of individual giant liposomes encapsulating identical genetic material. We followed the gene expression profiles from DNA and mRNA templates coding for different proteins. Although the average profiles of individual liposomes were similar to those measured in bulk solution, strong variability between individual liposomes was observed at both transcription and translation.