Nocardiajiangsuensis sp. nov., an actinomycete isolated from coastal soil
2016
A novel actinomycete strain, designated strain KLBMP S0027T, was isolated from a coastal soil collected from the coastal region of Lianyungang, Jiangsu Province, in east China, and was studied in detail for its taxonomic position. Comparative 16S rRNA gene sequence analysis showed that this strain belonged to the genus
Nocardia
and was most closely related to
Nocardia harenae
WS-26T (98.5 %),
Nocardia
asiatica NBRC 100129T (98.5 %),
Nocardia
abscessus NBRC 100374T (98.2 %),
Nocardia brasiliensis
NBRC 14402T (98.2 %) and
Nocardia
cyriacigeorgica NBRC 100375T (98 %), respectively. The strain showed a combination of chemotaxonomic and morphological properties typical of the genus
Nocardia
. The cell wall contained meso-diaminopimelic acid (type IV), and whole-cell sugars were arabinose, galactose, glucose and ribose. Strain KLBMP S0027T contained MK-8(H4ω-cycl) as the predominant menaquinone; C16 : 0, C18 : 1ω9c, C18 : 0 10-methyl (TBSA) and summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) were the major cellular fatty acids. The polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, one unknown glycolipid and two unknown lipids. Mycolic acids were detected. The G+C content of the DNA was 70.5 %. However, a combination of DNA–DNA hybridization and phenotypic data demonstrated that strain KLBMP S0027T could be clearly distinguished from the type strain of the most closely related species,
N. harenae
WS-26T. On the basis of the data presented from a polyphasic study, it was evident that this strain should be assigned to a novel species of the genus
Nocardia
, for which the name Nocardia jiangsuensis sp. nov. is proposed. The type strain is KLBMP S0027T (=CGMCC 4.7330T=KCTC 39691T).
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