Longitudinal study of HIV protease mutations in oral tissues of selected patients
2007
Twenty-nine HIV-1 infected patients from New Orleans were enrolled as a
cohort for this study over a four and one half year period. HIV-1
protease gene (pro) sequences were amplified using DNA isolated from
oral tissues (gingival cuff, buccal mucosa, tongue, palate) as well as
saliva and peripheral blood mononuclear cells (PBMC). PCR products were
directly sequenced using a combination of manual and automated methods,
and nucleotide sequences were translated using the universal genetic
code. Protein sequences obtained from independent amplifications of a
particular patient at a given time were consolidated into a single
consensus sequence and compared to HIV-1LAI to determine amino acid
replacements. The major findings were: 1) each patient had a signature
sequence that probably represented the predominant HIV–1
quasispecies; 2) over periods of 19 to 1673 days mutation patterns
remained relatively stable within a given patient; and 3) although
nearly 40% of the initial nonsynonymous replacements in the protease
signature sequences were mutations known to impart resistance to
protease inhibitors (PI), over time patients did not accumulate
additional PIR mutations.
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