SE33 variant alleles: Sequences and implications

Abstract U.S. population sample sets have been tested at the SE33 locus with PowerPlex ESX 17 and ESI 17 as well as AmpFlSTR NGM SElect, Investigator ESSplex SE, and the widely used "Polymeropoulos" primers to explore any concordance issues between kits possessing primers in different positions. A G→A mutation 68bp downstream of the repeat region has been detected in several samples that can cause a mobility shift in PowerPlex ESI 17 and ESSplex SE relative to PowerPlex ESX 17 and NGM SElect SE33 alleles. In addition, a C→T mutation 60bp downstream of the repeat region created the same mobility shift and allele dropout with the ESX 17 kit.