Callus Induction and Regeneration fromIn Vitroanther Culture of Rice (Oryza sativaL.)

The standardization of anther culture media for callus induction, and regeneration from anther derived callus of Azucena and Budda rice varieties. Anthers from panicles in which the distance between flag leaf and subtending leaf was 11cm in Azucena and 8 cm in Budda were used for anther culture. At this stage of development, anthers contained uni-nucleate pollen grains. Panicles were subjected to cold pretreatment of 5°C for 8 days. Callus induction frequency in different media combination ranged from 0.66% to 6.66% was observed in N6 medium supplemented with 1.0 mg−l,2, 4-Dichlorophenoxy acetic acid, 2.0 mg−l α-Naphthalene acetic acid and 0.5 mg−l Kinetin. In Azucena variety, highest callus induction (6.66%) while no callusing was found in Budda variety. Highest shoot regeneration (0.33%) from callus was observed in MS medium supplemented with 0.5 mg−l 6- Benzyl amino purine, 0.5 mg−l Kinetin and 80 mg−ladenine sulphate.