EFFECTS OF DIFFERENT PROSTAGLANDIN (PGF2ɑ) ANALOGUES DURING SYNCHRONIZATION OF OVULATION IN DAIRY BUFFALOES (Bubalus bubalis)

The study was conducted to determine the effects of prostaglandin analogues in buffaloes subjected to Fixed-Time AI (FTAI) using CIDR-Synch protocol. In Study 1, buffaloes were treated with GnRH and CIDR on day 0 and after the removal of CIDR on day 7, the animals were randomly assigned to different prostaglandin treatments: Dinoprost-Tromethamine (T1), Chloprostenol (T2), and D-Chloprostenol (T3). Blood samples were collected on days 7, 8, and 9 for progesterone assay. In study 2, buffaloes were subjected to the same treatments and FTAI protocol; signs of estrus were observed on the day of AI (day 10) and pregnancy diagnosis was performed on day 30-40. In the subset of animals per treatment, dominant follicle (DF) was measured at the time of AI. Results revealed progesterone concentrations of 1.67, 2.20 and 1.63 ng/ml in T1, T2 and T3, respectively, on day 7 and linear decline were observed thereafter with concentrations of 0.51, 0.59 and 0.70 ng/ml, respectively, on day 9. The average sizes of DF were 11.74, 13.3 and 13.02 mm, respectively. Estrus manifestation, DF diameter, and pregnancy rates were not significantly different among the groups. The work demonstrated the effectiveness of prostaglandin analogues in inducing luteolysis and estrus, and that chloprostenols are effective alternative luteolytic agents for FTAI in buffaloes.