IgG4/7 responses correlate with contraception in mares vaccinated with SpayVac

Abstract SpayVac ® is an immunocontraceptive vaccine based on porcine zona pellucida (pZP) antigens and uses a patented liposome formulation (VacciMax ™ or DepoVax ® ). It has delivered single-dose, long-lasting (4–10 years) immunocontraception in several species. Previous studies have demonstrated a positive correlation between levels of pZP antibodies produced and contraceptive effect; however, individual mares that were consistently infertile did not necessarily have the highest antibody titers. The objective of this study was to identify potential differences in specific immunoglobulin G (IgG) isotype responses among mares treated with SpayVac (VacciMax formulation) to improve our understanding of vaccine efficacy and potential management applications. We analyzed serum samples collected 1, 2 and 4 years post-vaccination from mares in another study that were continuously infertile or had foaled at least once during the 4-year period (n = 14 each). Additional samples from the continuously infertile mares were collected 5 years post-vaccination. A fluorescent bead-based assay was used to distinguish IgG isotype responses against pZP. IgG1 antibodies were generally higher in the infertile compared to the fertile mares, but only IgG4/7 antibodies were significantly higher in infertile mares during years 1 and 2 post-vaccination (p