Zea mays stem agricultural waste for in-vitro shoot propagation of Clinacantus nutans

The in-vitro shoot propagation performance of Clinacanthusnutans was observed under influenced of different concentration of Zea mays stem agricultural waste in powder and extracts forms. Phytochemical compounds were analyzed. The 4% extract generated optimum number of leaves and 1% extract was the best concentration to produce highest length of shoot. Thus, the used of Z. mays stem powder and extract effectively improved shoot propagation performance of C. nutans. But, the extract showed faster effect on C. nutansgrowth than the powder. The phytochemical screening showed positive result for the present of flavonoid, saponin and steroid. This result supported and shed light on how the Z. mays stem as additional nutrient contributes to the shoot propagation of C. nutans. This study also plays a vital role as basic study for further on-site large scale field application.