Evaluation of STR typings (TH01, TPOX and CSF1PO loci) using urine obtained from bladder cancer patients

Abstract We examined urine and blood DNA obtained from bladder cancer patients with commercially available STR kits such as those for TH01, TPOX and CSF1PO loci. These loci were amplified by PCR, and polymorphic alleles were visualized as bands on gels. The results were compared with each paired DNA. Microsatellite alternation was observed in 5 out of 52 (9.6%) urine DNAs from the patients with bladder cancer. These loss of heterozygosity (LOH) and microsatellite instability (MSI) were observed such as the combination of an intense and a faint band. Complete LOH causing incorrect typing results was not observed. We were able to avoid incorrect typing results when using urine DNA under careful observation.