Cell surface changes observed in MC-29 virus-infected chicken-embryo fibroblast (CEC) cultures.

By transmission and scanning electron microscopic studies of MC-29 virus-infected CEC cultures, C-type virus particles were detected in the medium of infected cultures, in infected cells and in intracellular vacuoles. Virus budding was also observed. In the scanning electron microscope (SEM) so-called blebs appeared on the surfaces of the infected cells, in a number proportional to the concentration of the virus preparation. On the basis of the size of the blebs, it could be considered that virus formation was taking place in their center. In control cells, neither viruses nor blebs were observed. This method will presumably be suitable for the titration of virus preparations. Modifications Particulieres De La Surface Cellulaire Observees Dans Les Cultures De Fibroblastes D'embryon De Poulet (Cec) Infectees Par Le Virus Mc-29 L'etude des cultures CEC infectees par le virus MC-29 au microscope electronique a transmission et a balayage a permis de deceler des particules virales de type C dans le milieu de culture, les cellules infectees et les vacuoles intracellulaires. On a egalement observe une production de virus. Au microscope a balayage, on a constate la presence, a la surface des cellules infectees, de sortes de vesicules dont le nombre etait proportionnel a la concentration de la preparation virale. D'apres la taille de ces vesicules, on peut considerer que le virus se forme en leur centre. Dans les cellules-temoins, on n'a observe ni virus, ni vesicules. Cette methode permettra probablement de titrer les preparations virales.