A quick method for cholesterol titration in complex in media

The titration of cholesterol is a difficult matter due to the large number of stages involved in the standardized method: extraction of the fat, then the unsaponifiable fraction, and sterol separation using TLC and gas chromatography analysis. The diversity of the products to be analyzed complicates fat extraction. The rapid method developed is based on placing the sample in solution or direct suspension in DMF, without prior fat extraction