Changes in release of prostacyclin by endothelial cells co-cultured with vascular smooth muscle cells under shear stress

Objective: To study the anti coagulation function of endothelial cells(ECs) co cultured with vascular smooth muscle cells(VSMCs). Methods: Exposed ECs co cultured with VSMCs to physiological laminar shear stress and PGI 2 was measured by Radioimmunoassay. Results: After exposed to shear stress, the peak release values and steady release values increased as the shear stress increased, the t 1/2 of peak release values decreased as the shear stress increased. Conclusion: The ability of anti coagulation of ECs co cultured with VSMCs increase under the shear stress. [