Differential Effect of Hypophysectomy on the Synthesis of β3-Glucuronidase and Other Androgen-Inducible Enzymes in Mouse Kidney

The levels of several androgen responsive enzymes including β3-glucuronidase, alcohol dehydrogenase, D-amino acid oxidase and arginase, were compared in kidneys of normal and hypo-physectomized female mice after treatment with testosterone. While hypophysectomy did not alter the basal level of glucuronidase, the androgen-mediated accumulation of kidney β3-glucuronidase was greatly decreased in hypophysectomized mice. Measurements of the rate of synthesis of glucuroni-dase showed that after androgen treatment the enzyme was synthesized in kidney of hypophysec-tomized mice at only 5% the normal rate. Glucuroni-dase activity in seven other organs was not appreciably affected by treatment with androgens or by hypophysectomy. Unlike the effect of hypo-physectomy on kidney glucuronidase, there was no reduction in the accumulation of alcohol dehydro-genase or D-amino acid oxidase in kidney of hypo-physectomized mice after androgen treatment. Hypophysectomy caused a large reduction in kidney arginase activity. Ho...