Preliminary study on genotype of Mycobacterium tuberculosis in some areas of Xinjiang by the multiple locus VNTR analysis

Objective To explore the multiple locus VNTR analysis (MLVA) of Mycobacterium tuberculosis. Methods It was selected that the data of the fifth TB epidemiological sampling survey in Xinjiang Uygur Autonomously Region from 2010 to 2011, and the genotyping was carried out by the classic 24 site method. The genetic clustering was analyzed by the BioNumerics5.0 database. The original strains of Mycobacterium tuberculosis, the annulus were dissolved and suspended bacterium in 400 μl TE, 80 ℃ 1 h inactivated, 12 000 r/min, the centrifugal 10 min, abandon supernatant, suspended bacterium in 600 μl TE again; multiple site tandem repeat sequence analysis. Results There were two genetic groups in Xinjiang: gene group Ⅰ and gene group Ⅱ, 66 strains in genetic group Ⅰ (66.7%), and 33 strains in gene strain Ⅱ (33.3%). gene growp I was Beijing genotype strains 65 different genotype from gene group of 66 strains of Mycobacterium tuberculosis Ⅰ, 2 strains of Mycobacterium tuberculosis belong to the same cluster, clusters rate was 1.5%, It was different that the gene group of Ⅱ 33 of Mycobacterium tuberculosis strain of MLVA pictures, clusters rate was 0. Conclusion Mycobacterium tuberculosis strains exist obvious gene polymorphism in Xinjiang, with the majority of Beijing genotype strains, and a certain proportion of strains are not Beijing genotype, we should strengthen the monitoring and management for major epidemic strains. Key words: Tuberculosis; Mycobacterium tuberculosis; Multiple locus VNTR analysis; Gene polymorphism; Xinjiang areas