ANTIOXIDANT AND ANTICOAGULANT ACTIVITY OF NOVEL N- SUBSTITUTED 4 METHYL 5,7 DI HYDROXYL COUMARIN AND ITS ESTER DERIVATIVES

Some novel N- Substituted 4 methyl 5,7 di hydroxyl coumarins were synthesized efficiently in two methods general synthesis and microwave method in 4 steps. Both the methods 12 compounds were synthesized. The synthesized compounds were identified by IR, NMR spectroscopic techniques. The present investigation deals with the synthesized compounds possessing good anti-oxidant and anti-coagulant activity. The anti-oxidant activity is carried out by DPPH method, Nitric oxide scavenging method and Hydrogen peroxide method. Compounds V and VI shown the maximum radical scavenging activity in DPPH method. Compounds V and II has shown the maximum activity in Hydrogen peroxide method. In Nitric oxide scavenging activity the compounds VI and VII have shown maximum activity. In in vivo Nitric oxide radical scavenging activity method the compounds effectively reduced the generation of nitric oxide from sodium nitroprusside by using Griess reagent. In anti-coagulant activity the Clotting time, bleeding time and Prothrombine time also observed. The compounds V and VI are more active in Clotting time. The compounds V and IV are more active in bleeding time. The compounds I and V are more active.