Influence of Urea Alone or Combined with Fish Solubles, Fish Meal, or Feather Meal in Liquid Supplement with and Without L-Carnitine on Performance and Ruminal and Metabolic Parameters of Weanling Calves

Using growing calves (n = 368) grazing mature forage in three 84-d experiments, one objective was to compare the effects of feeding molasses-based liquid supplements containing urea (U) alone or combined with fish solubles (FS), fish meal (FM), or feather meal (FE) on performance, and blood metabolites. Another objective was to evaluate the effects on these criteria of adding carnitine to these supplements to provide 1 g·h−1·d−1 L-carnitine. Including FM or FE in the mixture improved (P<0.05) supplement consumption in two experiments and improved growth rate in all experiments. Carnitine improved the growth rate when U was the only protein source, but reduced growth rate when FM was included in the liquid supplement (P<0.05). During the growth phase, plasma ammonia N (PAN) was higher (P<0.05) when U was the only protein source as compared with FM or FE. Carnitine did not influence these metabolic parameters. During the metabolic phase of each experiment, calves similar to those used in the growth phase were dosed via stomach tube with 3 g liquid supplement/kg BW. Ruminal and blood samples were collected at 0, 0.5, 1, 2, and 4 h after dosing. Ruminal ammonia N, PAN, and plasma urea N (PUN) concentrations were higher (P<0.05) when calves were dosed with U or FS supplement than when dosed with FM or FE supplements. Calves dosed with liquid supplement containing carnitine had lower (P<0.05) concentrations of ruminal ammonia N and PAN. Carnitine reduced (P<0.05) PUN in one experiment and increased (P<0.05) PUN in another. Branched-chain volatile fatty acids were increased (P<0.05) by dosing with FS or FM supplements. Including ruminally undegradable protein sources (FM or FE) in liquid supplement improved intake and performance and was related to reduced ruminal ammonia N, PAN, and PUN. Carnitine fed in liquid supplement also reduced ruminal ammonia N and PAN concentrations.