Additional file 1: Table S1. of Deciphering functional diversification within the lichen microbiota by meta-omics
2017
Metadata and basic information related to the DNA/RNA-based omic approaches. Table S2: Primer sets for eukaryotic and prokaryotic rRNA gene fragment amplification. Bold: T7 promoter sequence; blue: enhancer sequence. Table S3: Taxonomical assignments. Fig. S1: Molecular fingerprints of 16S rRNA gene fragments from total DNA (Tot DNA) from the lichen, isolates from the lichen thalli (L. pulmonaria) and control strains (E. coli, S. aureus, P. aeruginosa). Fig. S2: Phylogenetic tree of bacteria-strains within the phylum Verrucomicrobia. The tree was constructed from an evolutionary distance matrix based on the neighbor-joining method [59]. The phylogenetic tree was constructed with 1000 seeds and 1000 bootstraps with the neighboring-joining method [59] using clustalX2 [45] and phylip [24]. Fig. S3: Common and unique OTUs among three omics approaches based on 16S rRNA gene fragment sequences with a genetic distance of 3%.
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