Investigation of the interaction between FTO and 3-substituted 2-aminochromones by spectroscopy and molecular modeling

The fat mass and obesity-associated protein is a potential target for anti-obesity medicines. In the present work, the interaction between our synthesized 3-substituted 2-aminochromones and fat mass and obesity-associated protein were investigated using fluorescence spectroscopy, ultraviolet–visible spectroscopy and molecular modeling approach. Fluorescence spectroscopy showed that the fluorescence of fat mass and obesity-associated protein can be quenched by these compounds with a static quenching procedure. In addition, the thermodynamic parameters obtained from the fluorescence data showed that the hydrophobic force played a major role in stabilizing the complexes. Ultraviolet–visible spectroscopy, synchronous fluorescence spectroscopy and three-dimensional fluorescence spectroscopy indicated that these compounds can induce some conformational changes of fat mass and obesity-associated protein.