SEED PROTEINS ANALYSIS OF FESTUCA SERIES PSAMMOPHILAE M. PAWLUS AND OVINAE M. PAWLUS (POACEAE)

2020 
According to the type of leaf anatomy, narrow-leaved fescues could be divided to three main groups: species with leaves as in F estuca rubra (sclerenchyma strands small, numerous), type F. valesiaca (3 main and big sclerenchyma strands + adventive) and type F. ovina (sclerenchyma ring). The species of the last group were chosen in our study. The seed proteins variation in natural populations of Festuca vaginata , F. psammophila , F. pallens , F. polesica and F. ovina from Ukraine and Bulgaria was examined by means of polyacrylamide (PAGE) and sodium dodecyl sulphate (SDS-PAGE) electrophoresis. These fescues exhibit high variability and overlap of morphological and anatomical characters, including diagnostic ones, leading to identification difficulties and uncertainties in their taxonomy. The purpose of the present study was to analyze seed protein composition in order to reveal systematic relationships among the above mentioned taxa of genus Festuca . The results showed that F. vaginata , F. psammophila and F. polesica are closely related. The species F. pallens was relatively distant from the above mentioned three species. The specific position of F. pallens is confirmed also by its ecological characteristics– it is the only species growing on carbonate rocks, while the rest of taxa are typical psammophytes. The results showed distinct differences between Ukrainian populations of F.  p allens and F. psammophila and confirmed occurrence of latter in Ukrainian Roztocha. The cluster patterns were somewhat inconsistent in regard to different types of proteins (PAGE, SDS-PAGE). The only exception is F. ovina , which always occupies a separate position. This confirms its remote position within the studied group of the genus Festuca and supports the view to consider F. ovina s.str. as a separate series Ovinae among the species with sclerenchyma ring. Whereas all other studied species ( F . vaginata , F. psammophila , F. pallens , F. polesica ) should be considered as a separate series of closely related taxa – series Psammophilae .
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