6‴-Feruloylspinosin alleviated beta-amyloid induced toxicity by promoting mitophagy in Caenorhabditis elegans (GMC101) and PC12 cells

Abstract This study aims to investigate the neuroprotective effects of 6‴-feruloylspinosin (6-FS), one of the main active flavonoid components in Sour Jujube seeds, on beta-amyloid (Aβ) protein transgenic Caenorhabditis elegans (GMC101) and PC12 cells, and determine the molecular mechanism of its action. We found that 6-FS could ameliorate the progression of the Alzheimer's disease (AD) phenotype by delaying the aging, decreasing the rate of paralysis, enhancing resistance to heat stress, and increasing the chemotaxis ability, and promotes autophagy activity though autophagy/lysosome pathway in GMC101. Furthermore, 6-FS reduced Aβ-induced toxicity by inhibiting the deposition of Aβ and the aggregated proteins, increasing the level of mitophagy in PC12 through promoting the expression of Pink1/Parkin in the mitophagy pathway. Our findings suggest that 6-FS may be used as a medicinal supplement for treating AD.