Anti-HIV-1-virus tuberculosis gene vaccine, and preparation method and application thereof

The invention relates to an anti-HIV-1-virus tuberculosis gene vaccine composed of a vector. An exogenous target gene is inserted into the vector. The exogenous target gene comprises a gene of a capsid protein p24 sourced from a HIV-1NL4-3 strain. T cell epitope polypeptide genes MPT6476-84, Ag85A242-250, Ag85B184-192 and TB10.474-82 from mycobacterium tuberculosis antigen are embedded in the capsid protein p24 gene. The invention also provides a preparation method and an application of the anti-HIV-1-virus tuberculosis gene vaccine. When the gene vaccine is used in intramuscular injection for immunizing mice, Th1-type immune response and specific killing response secreting high-level IFNgamma aiming at tuberculosis antigens can be induced, and specific serum IgG and T-cell immune response aiming at HIV-1 antigen p24 can be induced. Therefore, the vaccine can be used for preventing tuberculosis and inhibiting HIV.