Assessment of Therapeutic Effect of Buffered Phosphorus in Post Parturient Haemoglobinuria (PPH) in Buffaloes

The investigation was carried out to assess the erythrocytic oxidative stress indices such as erythrocytic malondialdehyde (MDA), reduced glutathione (GSH) and erythrocytic fragility after treatment of buffaloes affected with Post-parturient haemoglobinuria with buffered phosphorus, inosine and sodium pyruvate in place of conventional treatment regimen. The study was carried out on six clinical cases of PPH. Blood samples were collected on day zero (before treatment) and 3 to 4 days after treatment (i.e. after recovery). The biochemical parameters improved significantly following treatment as compared to pre-treatment level. A significant (P<0.05) improvement was observed on day 4 with respect to erythrocytic MDA which decreased from 528.69±0.76 n moles/ml RBC to 290.44±2.05 n moles/ml RBC (after treatment). Activities of antioxidant enzymes, viz. reduced glutathione increased significantly (P<0.05) was reduced from 32.40±0.92 to 53.53±0.71 mg%. The erythrocytic osmotic curve shifted toward right showing decrease in percent haemolysis after treatment. Thus the decline of MDA levels to within normal limits and appreciable elevation of reduced GSH levels following treatment with buffered phosphorus, inosine and sodium pyruvate revealed its desired and antioxidant effect in haemoglobinuric buffaloes.