Construction of SSH-cDNA Library for Cytoplasm Male Sterility Lines and Maintainer Lines in Pepper

A forward subtracted cDNA library contained expression genes of pepper cytoplasm male sterility maintainer lines,was constructed by using suppression subtractive hybridization technique. The pepper cytoplasm male sterile lines A1 and A5 were used as drivers,and the corresponding maintainer lines B1 and B5 as testers. Based on the 189 positive monoclones selected from the subtracted library,42 expressed sequence tags(ESTs) were identified by DIG Nonradioactive Nucleic Acid Labeling and Detection System as well as sequencing. The BLAST analysis showed that 35 ESTs had gene potential functions,including 26 ESTs that are homologous to known genes,and 9 ESTs without homology. Most of the homologous gene functions are related to the basic material metabolism,stress defense,signal transduction;the 7 ESTs without BLAST results might imply some new genes.