The in vitro Antibacterial Efficacy of Persian Green Tea Extract as an Intracanal Irrigant on Enterococcus faecalis Biofilm

Introduction: The aim of this study was to compare the antibacterial effect of Persian green tea extract (GTE) and 2.5% sodium hypochlorite (NaOCl) against Enterococcus faecalis ( E. faecalis ) as an intracanal irrigant. Methods and Materials: Thirty freshly extracted teeth were instrumented and sectioned into mesial and distal segments. The specimens were put into wells containing 2 mL of E. faecalis -containing medium. After 3 weeks, the specimens were removed and divided randomly into three groups ( n =20). Each group was exposed to 3 mL of different irrigants for 3 min. Groups 1, 2 and 3 were irrigated with GTE, 2.5% NaOCl and normal saline, respectively. Biofilm formed in the middle third of the root canal was carved by sterile scalpel and cultured in Mueller-Hinton medium. Number of colony forming units (CFU) was counted on each plate. In addition, antimicrobial activity of the irrigants was evaluated by the agar disc diffusion test. The diameter of inhibition zone (IZ) around each irrigant was evaluated. The Kruskal-Wallis and Dunn tests were used to analysis the data. Results: While in NaOCl group no bacterial colonies were observed, the mean number of E. faecalis in GTE and control groups were 275±74 CFU/mL ( P <0.001) and 119×10 8 ±11×10 8 ( P <0.001), respectively. The mean of IZ in NaOCl and GTE groups were 24.35±0.78 and 6.9±0.87 mm, in order of appearance ( P <0.001). Zone of inhibition was not observed around the control group ( P <0.001). Conclusion: This research highlighted the potential role of plant extracts in antimicrobial root canal irrigation protocol. Keywords: Biofilm; Enterococcus faecalis ; Green Tea Extract; Root Canal Irrigant; Sodium Hypochlorite