Expression d'une protéine liée au sexe, la peptidase-1, chez des Pleurodcles femelles soumises à un vol orbital et recherche d'anomalies génétiques dans leurs descendances

The "Experience Triton" embarked on board of Biosatellite Cosmos 2229 (or Bion 10) pooled several experiments engaged by different teams. Our laboratories have to study in the amphibian urodele Pleurodeles waltl : 1- the expression of a sex-linked enzyme, the peptidase-1, in embarked and synchrone control females. The electrophoretical patterns of the enzyme in ovary, gut and muscles of flight samples were similar to erythrocyte controls but they were slightly different for kidney and heart. 2- the detection of possible genetic abnormalities in the offspring of females submitted to space environment. Embarked females and synchrone females were used as donors of ovaries both to castrated adult females and juveniles males. In may 1994, one recipient female and 6 recipient males are alive in may 1994. Laparotomy of the host animals allowed to oversee the success of the graft for all the surviving animals excepted one male which had received an ovary of an embarked female.Because of the slow development of ovaries in Pleurodeles , the success of this space experiment cannot be ascertain before the reproduction of these animals which will conduct in some months