Purification technology of benzylbenzoate glycosides from Curculigo orchioides Gaertn. with D101 macroporous resin

Objective:To optimize the purification technology of benzylbenzoate glycosides(BBG)from Curculigo orchioides Gaertn.with D101macroporous resin.Methods:With the absorption quantity and desorption rate of curculigoside and curculigoside B as indexes,the static and dynamic absorption and desorption properties of BBG from Curculigo orchioides Gaertn.with D101macroporous resin were investigated.Results:The optimal purification conditions for absorption of BBG from Curculigo orchioides Gaertn.with D101macroporous resin were as follows:absorption time was 8h,pH of sample liquid was 6,pH of ethanol was 8,temperature of absorption and desorption was room temperature,the optimal concentration of BBG in sample liquid was 0.22mg/ml,flow rate of absorption was 2.5Bv/h,the elution solvent was 30%ethanol and the dosage was 12Bv,the concentration of BBG in sample liquid for second purification was 0.17mg/ml.Conclusion:This purification process was simple and stable,and the content of BBG could be increased from 0.022%to more than 1.60%,the content could reach 5.08%after second purification.