Preparation method of human glycated hemoglobin

The invention discloses a preparation method of human glycated hemoglobin. The preparation method comprises the following steps: extracting erythrocyte from a blood sample, washing the obtained erythrocyte to release the hemoglobin in erythrocyte, and finally separating and purifying the obtained hemoglobin. The conventional technology is difficult to obtain high purity glycated hemoglobin and has the disadvantages of low recover rate and low flux. According to the provided preparation method, blood samples of healthy adult volunteers and diabetes patients are taken as the raw materials, and a free flow isoelectric focusing mode is adopted to prepare human glycated hemoglobin efficiently. The provided method can obtain human glycated hemoglobin with a purity of 96% or more, and moreover, the recovery rate of human glycated hemoglobin can reach 98% or more and is 30% higher than the recovery rate which has been reported. The human glycated hemoglobin has a wind market prospect, can be used as a diabetes detection standard, and can be used to prepare polyclonal antibody and antigen of monoclonal antibody.