Yeast Surface Display System for Facilitated Production and Application of Phage Endolysin
2020
Bacteriophage
endolysin is one of the potential alternatives of
conventional antibiotics, but the intrinsic limitations of the bacterial
expression system may undermine the comprehensive application of this
therapeutic protein. To circumvent such limitations, we adopted a
yeast surface display system as a novel expression platform for endolysin.
Endolysin LysSA11 from staphylococcal phage SA11 was expressed and
surface-displayed in Saccharomyces cerevisiae to
exhibit sufficient antimicrobial activity against Staphylococcus
aureus. Without any protein isolation or purification procedures,
we showed that direct treatment of LysSA11-displaying yeast cells
could accomplish a 5-log reduction of viable Staphylococcus
aureus within 3 h. Furthermore, the surface-displayed LysSA11
exhibited superior stability over the soluble form of purified LysSA11
during 14 days of storage in a refrigerated environment. We suggest
that the yeast surface display system is an efficient, stable, and
straightforward platform for the production and antibacterial applications
of endolysin.
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