Effects of CHI3L1 gene silenced by siRNA on proliferation and invasion of human gallbladder carcinoma cells
2017
Objective
To investigate the effects of CHI3L1 gene silenced by siRNA upon the proliferation and invasion of human gallbladder carcinoma cells.
Methods
The CHI3L1 gene of the human gallbladder carcinoma cell GBC-SD was silenced by slow-virus infection and siRNA interference technique (siRNA group). And negative control group (siRNA-NC group) and blank control group (GBC-SD group) were established. The cell absorbance values (A450) among three groups were detected by CCK-8 assay. The quantity of membrane-penetrating cells was detected by Transwell cell migration and invasion assays. The cellular data among three groups were compared by one-way analysis of variance and LSD-t test.
Results
The A450 at 24, 48, 72 h in the siRNA group was respectively 0.87±0.16, 0.47±0.21 and 0.52±0.20, significantly lower than 1.20±0.08, 1.34±0.15 and 1.58±0.21 in the GBC-SD group (LSD-t=-4.061, -8.483, -7.698; P<0.05). Transwell cell migration assay revealed that, the quantity of membrane-penetrating cells at 24 h in the siRNA group was 31.0±2.4, significantly less than 92.0±7.2 in the GBC-SD group (LSD-t=-8.126, P<0.05). Transwell cell invasion assay revealed that, the quantity of membrane-penetrating cells at 24 h in the siRNA group was 29.0±3.0, significantly less than 76.0±5.2 in the GBC-SD group (LSD-t=-7.883, P<0.05).
Conclusions
CHI3L1 gene silenced by siRNA can significantly inhibit the proliferation, migration and invasion capabilities of human gallbladder carcinoma cells.
Key words:
Gallbladder neoplasms; RNA, small interfering; Neoplasm invasiveness; Cell migration assays
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