Ribonucleic acid interference (RNAi) for inhibiting foot and mouth disease virus replication and preparation method of RNAi

The invention discloses ribonucleic acid interference (RNAi) for inhibiting foot and mouth disease virus replication and a preparation method of RNAi. The RNAi comprises a small interfering RNA (siRNA) sequence. The preparation method comprises the steps of constructing a short hairpin RNA (shRNA) slow virus expression vector, preparing replication-defective slow virus, infecting slow virus pig kidney (PK)-15 cells (pig kidney cells) and the like. The invention also discloses a method for verifying the effect of inhibiting foot and mouth disease virus replication. The RNAi sequence has the obvious effect of inhibiting the foot and mouth disease virus replication on sensitive cells. According to the invention, the exploration of RNA interference on in vitro and vivo replication of foot and mouth disease virus is carried out, a slow virtue mediated stable-integrated RNA interfering technology for conserved gene segments of a targeted foot and mouth disease virus genome, and transgenic animals with the siRNA of the targeted foot and mouth disease virus are hopeful to construct. According to the invention, necessary experimental data is accumulated for gene function study of RNAi applied to the foot and mouth disease virus and prevention and treatment of foot and mouth disease, and early-stage preparation is provided for disease resistance breeding of animals.