BÚSQUEDA DE BIOMARCADORES PARA LA DISCRIMINACIÓN DE LAS VARIEDADES DE KAÑIWA (Chenopodium pallidicaule Aellen) “CHILLIWA” Y “RAMIS” POR MEDIO DE METABOLÓMICA NON-TARGETED

“Kaniwa" (Chenopodium pallidicaule Aellen) is a very nutritious Andean grain characterized by its high protein content and good amino acid profile. The diversity of this species has been characterized by agro-morphological and genomic studies, which are not completely efficient to discriminate between accessions and varieties. Metabolomics is currently used as an additional tool for species delimitation. In this sense, in the present work a nontargeted metabolomic analysis of two varieties of kaniwa (“Ramis” and “Chilliwa”) from the department of Puno, Peru was carried out. For the analysis, ultra-high performance liquid chromatography coupled to high resolution mass spectrometry with Orbitrap analyzer (UHPLC-HRMS) was used. The MSDIAL and MSFINDER software were used to identify the metabolites. The chemical profiles obtained were summarized in a single table for statistical analysis with the SIMCA P software. The OPLS-DA statistical model allowed the classification of both varieties of kaniwa and the S-plot graph showed the main putative biomarkers for discrimination of both varieties. The metabolite delfinidine-O-(6''-O-α- ramnopyranosyl-β-glucopyranoside) was identified as one of the main biomarkers for kaniwa “Ramis”.