Rapid propagation method for tissue cultivation of dendrobium candidum stem

2013 
The invention relates to a rapid propagation method for tissue cultivation of a dendrobium candidum stem, belonging to the technical field of artificial cultivation of dendrobe. The method comprises the following steps of preparing an explant, inducing axillary bud and protocorm, conducting propagation cultivation, and rooting and seeding cultivation. The rapid propagation method has the beneficial effects that the technical problems of insufficient explant sterilization, high pollution rate, incapability of conducting axenic cultivation, slow protocorm differentiation, less differentiation, long propagation period, high production cost and the like can be well solved. Compared with the prior art, the rapid propagation method has the advantages that rapid propagation can be achieved; and the propagation period can be reduced by over 60 days. In addition, according to the rapid propagation method, seeding genetic traits are stable, the variation is small, the variety is pure, and the parent characteristics and features can be well kept. Thus, excellent seeding guarantee is provided for development of plant industry of dendrobium candidum, and the rapid propagation method has good economic benefit and social benefit.
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