Results Multi-Laboratory Trial ISO/CD 22184 – IDF/WD 244: Milk and milk products – Determination of the sugar contents – High performance anion exchange chromatography method with pulsed amperometric detection (HPAEC-PAD)

BACKGROUND In this international Multi-Laboratory Trial (MLT) the precision data and the accuracy of the ISO/CD 22184 IDF/WD 244 analytical method for the quantification of 6 different mono- and disaccharides (galactose, glucose, fructose, sucrose, lactose, and maltose) in milk and milk products with high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) are established. OBJECTIVE Thirteen different laboratories participated (located in New Zealand, the United States, and Europe). The study was divided into two parts. First, the learning/familiarization part in which the participants got experienced with the method. After successfully completing this part, the participants were allowed to continue with the second part of this trial: the analyses of the seven blind MLT duplicate samples. METHOD The selected seven MLT samples comprise five MUVA reference dairy samples, one NIST-certified infant formula and one industrial sample. In the Tables S-1 to S-6 the results of this MLT study have been summarized per sugar. There is a very good agreement between the MUVA and NIST-certified sugar contents in the MLT samples and in the MLT established sugar contents in those MUVA and NIST samples. RESULTS This demonstrates that the accuracy of the newly developed HPAEC-PAD method is very good. CONCLUSIONS Certificates of analysis were available for both the MUVA samples and the NIST sample. There is a very good agreement between the by MUVA- and NIS-certified sugar contents in the MLT samples and the corresponding in the MLT established sugar contents. This demonstrates that the accuracy of the HPAEC-PAD method is very good. In the IDF/ISO Analytical Week in June 2019 in Prague the report on the MLT study were discussed and adopted.