Bioconversion of lignocellulosic substrates for the production of polyhydroxyalkanoates

Abstract Lignocellulose, the most abundant and renewable carbon resource, is untapped due to its protective lignin shield. Biological pretreatment employs white rot fungi (WRF) to remove lignin and expose the cellulose/hemicellulose layers for exploitation. The present work was conducted to explore the possibility of biologically pretreating lignocellulose with WRF and using the sugars, liberated from this process, as renewable and inexpensive feedstock for the production of polyhydroxyalkanoates (PHA). Pleurotus ostreatus MTCC 142 (P142) was used to bring about bio-delignification of lignocellulose under solid state fermentation (SSF) conditions. The hydrolysates thus obtained were used for bioconversion to PHA, using Bacillus megaterium Ti3 under submerged fermentation (SmF) conditions in a nitrogen-deficient medium. From the lignocellulosic substrates used, the highest sugar yields, post bio-delignification, were obtained from corn husk (6 mg/mL). Production of PHA from pure sugars revealed a preference for xylose with 50% PHA accumulation. The pretreated corn husk (CH) hydrolysate yielded 1 g/L PHB with an accumulation of 57.8% after 48 h, comparable to that obtained using xylose based medium and significantly higher (p