Association between ACE, eNOS, FVII and ICAM-1 gene polymorphism and coronary heart disease with abnormal humour syndrome in Uighur medicine.

This paper studies the gene polymorphism of angiotensin-converting enzyme(ACE) gene,nitric oxide synthase(eNOS) gene,coagulation factor VII(FVII) gene and intercellular cell adhesion molecules-1(ICAM-1) of abnormal humour syndrome patients with coronary heart disease(CHD).The four types of gene related to CHD were prepared by polymerized chain reaction -restriction fragment length polymorphism(PCR-RFLP) in 92 cases of CHD patients and 30 healthy subjects.Test results show no significant difference in the genotype distribution and the allele frequencies of ACE gene,FVII gene,ICAM-1 gene in various types of abnormal humour syndrome patients with CHD as compared to those of healthy subjects(p0.05).There are significant differences in the genotype distribution and the allele frequencies of eNOS gene in three types of abnormal humour syndrome patients with CHD(abnormal Sapra syndrome patients,abnormal Savda syndrome patients,abnormal Khan syndrome patients) as compared to those of healthy subjects(p0.05).However there are no significant difference in the genotype distribution and the allele frequencies of eNOS genein abnormal Balgham syndrome patients as compared to those of healthy subjects(p0.05).It is concluded that the genotype distribution and the allele frequencies of ACE gene,FVII gene,ICAM -1 gene are not correlated with various types of abnormal humour syndrome patients with CHD.But the genotype distribution and the allele frequencies of eNOS gene are closely correlated with three types of abnormal humour syndrome patients with CHD(abnormal Sapra syndrome patients,abnormal Savda syndrome patients,abnormal Khan syndrome patients).The genotype distribution and the allele frequencies of eNOS gene are not correlated with abnormal Balgham syndrome patients with CHD.